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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ESRRB Monoclonal antibody

ESRRB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E10C1

Cat no : CL488-66818

Synonyms

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite® Plus 488-conjugated ESRRB Monoclonal ant (CL488-66818)

    IF Staining of Caco-2 using CL488-66818

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 ESRRB antibody (CL488-66818, Clone: 3E10C1 ) at dilution of 1:200, Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red).

  • Flow cytometry (FC) experiment of Caco-2 cells using CoraLite® Plus 488-conjugated ESRRB Monoclonal ant (CL488-66818)

    FC experiment of Caco-2 using CL488-66818

    1x10^6 Caco-2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ESRRB Monoclonal antibody (CL488-66818, Clone:3E10C1)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66818 targets ESRRB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESRRB fusion protein Ag18626 相同性解析による交差性が予測される生物種
Full Name estrogen-related receptor beta
Calculated molecular weight 508 aa, 56 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC131517
Gene symbol ESRRB
Gene ID (NCBI) 2103
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ESRRB antibody CL488-66818Download protocol
FC protocol for CL Plus 488 ESRRB antibody CL488-66818Download protocol
Standard Protocols
Click here to view our Standard Protocols