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- KD/KO Validated
ESYT1 Monoclonal antibody
ESYT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2C12E4
Cat no : 67688-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HSC-T6 cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
67688-1-Ig targets ESYT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ESYT1 fusion protein Ag16840 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 62 (C2 domain containing), member A |
| Calculated molecular weight | 1104 aa, 123 kDa |
| Observed molecular weight | 123 kDa |
| GenBank accession number | BC004998 |
| Gene symbol | ESYT1 |
| Gene ID (NCBI) | 23344 |
| RRID | AB_2882881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| IF protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shihab (Verified Customer) (09-17-2021) | This antibody was used in a western blot application to identify recombinant Esyt1 prescence. Using 1:500 at the first attempt, this antibody was able to successfully provide a band at the correct size. Furthermore, the band was clean which was an added bonus. I will be reducing the concentration of antibody used and also trying this antibody in other applications as well as primary cell lysate
|
