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  • Featured Product
  • KD/KO Validated

ESYT1 Monoclonal antibody, PBS Only

ESYT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C12E4

Cat no : 67688-1-PBS

Synonyms

FAM62A, Extended synaptotagmin-1, Extended synaptotagmin 1, E-Syt1, E Syt1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis using 67688-1-Ig (same clone as 67688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (ESYT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis using 67688-1-Ig (same clone as 67688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis of HSC-T6 using 67688-1-Ig (same clone as 67688-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ESYT1 Monoclonal antibody (67688-1-Ig)

    IF Staining of U2OS using 67688-1-Ig (same clone as 67688-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ESYT1 antibody (67688-1-Ig, Clone: 2C12E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67688-1-PBS targets ESYT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ESYT1 fusion protein Ag16840 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 62 (C2 domain containing), member A
Calculated molecular weight 1104 aa, 123 kDa
Observed molecular weight123 kDa
GenBank accession numberBC004998
Gene symbol ESYT1
Gene ID (NCBI) 23344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.