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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ETV4 Recombinant antibody

ETV4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241042E10

Cat no : CL488-83891-6

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated ETV4 Recombinant ant (CL488-83891-6)

    IF Staining of A431 using CL488-83891-6

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 ETV4 antibody (CL488-83891-6, Clone: 241042E10 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83891-6 is ETV4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ETV4 fusion protein Ag0984 相同性解析による交差性が予測される生物種
Full Name ets variant 4
Calculated molecular weight 54 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC007242
Gene symbol ETV4
Gene ID (NCBI) 2118
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ETV4 antibody CL488-83891-6Download protocol
Standard Protocols
Click here to view our Standard Protocols