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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EVL Polyclonal antibody, PBS Only

EVL Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13484-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using EVL Polyclonal antibody (13484-1-AP)

    WB analysis of Jurkat using 13484-1-AP (same clone as 13484-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13484-1-PBS targets EVL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVL fusion protein Ag4302 相同性解析による交差性が予測される生物種
Full Name Enah/Vasp-like
Calculated molecular weight 418 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC032358
Gene symbol EVL
Gene ID (NCBI) 51466
RRIDAB_10644121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).