Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66537-1-Ig targets EXOC3L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EXOC3L2 fusion protein Ag26912 相同性解析による交差性が予測される生物種 |
| Full Name | exocyst complex component 3-like 2 |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC104947 |
| Gene Symbol | EXOC3L2 |
| Gene ID (NCBI) | 90332 |
| RRID | AB_2881900 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q2M3D2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOC3L2 antibody 66537-1-Ig | Download protocol |
| IF protocol for EXOC3L2 antibody 66537-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Genet Biallelic mutations in EXOC3L2 cause a novel syndrome that affects the brain, kidney and blood. |