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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using EXOC3L2 Monoclonal antibody (66537-1-Ig)

    WB analysis of HUVEC using 66537-1-Ig (same clone as 66537-1-PBS)

    HUVEC, HEK-293 and U-251 cells were subjected to SDS PAGE followed by western blot with 66537-1-Ig (EXOC3L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66537-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using EXOC3L2 Monoclonal antibody (66537-1-Ig)

    IF Staining of HUVEC using 66537-1-Ig (same clone as 66537-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using EXOC3L2 antibody (66537-1-Ig, Clone: 3C1C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66537-1-PBS in a different storage buffer formulation.

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EXOC3L2 Monoclonal antibody, PBS Only

EXOC3L2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66537-1-PBS
Clone No.3C1C2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EXOC3L2, XTP7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66537-1-PBS targets EXOC3L2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXOC3L2 fusion protein Ag26912 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 3-like 2
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC104947
Gene Symbol EXOC3L2
Gene ID (NCBI) 90332
RRIDAB_2881900
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ2M3D2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.