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  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody, PBS Only

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11178-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EXOSC10 Polyclonal antibody (11178-1-AP)

    WB analysis of HeLa using 11178-1-AP (same clone as 11178-1-PBS)

    WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells. This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using EXOSC10 Polyclonal antibody (11178-1-AP)

    IP experiment of MCF-7 using 11178-1-AP (same clone as 11178-1-PBS)

    IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug. This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EXOSC10 Polyclonal antibody (11178-1-AP)

    IHC staining of human breast cancer using 11178-1-AP (same clone as 11178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using EXOSC10 Polyclonal antibody (11178-1-AP)

    IF Staining of NIH/3T3 using 11178-1-AP (same clone as 11178-1-PBS)

    Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11178-1-PBS targets EXOSC10 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag1666 相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 98 kDa
Observed molecular weight100 kDa
GenBank accession numberBC039901
Gene symbol EXOSC10
Gene ID (NCBI) 5394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.