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KD/KO Validated

EYA1 Polyclonal antibody

Name: EYA1 Polyclonal antibody
Brand: Proteintech
SKU: 22658-1-AP

EYA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22658-1-AP

Synonyms

EC:3.1.3.16, BOR, BOP

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 22658-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 22658-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 22658-1-AP
IP result of anti-EYA1 (IP:22658-1-AP, 4ug; Detection:22658-1-AP 1:4000) with HepG2 cells lysate 2600 ug.
IHC staining of human kidney using 22658-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 22658-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 22658-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA1 antibody (22658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 22658-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse colon tissue, HuH-7 cells, NCCIT cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 9 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

22658-1-AP targets EYA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EYA1 fusion protein Ag18489 相同性解析による交差性が予測される生物種
Full Name	eyes absent homolog 1 (Drosophila)
Calculated molecular weight	592 aa, 65 kDa
Observed molecular weight	61 kDa and 65 kDa
GenBank accession number	BC121799
Gene symbol	EYA1
Gene ID (NCBI)	2138
RRID	AB_2879145
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

Protocols

Product Specific Protocols
WB protocol for EYA1 antibody 22658-1-AP	Download protocol
IHC protocol for EYA1 antibody 22658-1-AP	Download protocol
IF protocol for EYA1 antibody 22658-1-AP	Download protocol
IP protocol for EYA1 antibody 22658-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Rep YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis Authors - Qian Fang View Article
human	IHC,IF	Front Oncol SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1. Authors - Deguang Kong View Article
human	WB,IHC	Oncotarget Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma. Authors - Joshua Jiawei Zhou KD Validated View Article
human	WB	Stem Cell Res Ther Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model. Authors - Qiuxia Cui View Article
human	IHC	Clin Breast Cancer Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis. Authors - Qiuxia Cui View Article
rat	WB,IP	J Mol Neurosci MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling. Authors - Jin Gao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 22658-1-AP

WB analysis of mouse colon using 22658-1-AP

IP experiment of HepG2 using 22658-1-AP

IHC staining of human kidney using 22658-1-AP

IHC staining of human kidney using 22658-1-AP

IF Staining of HeLa using 22658-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis

SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1.

Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma.

Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.

Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.

MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling.