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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22658

Synonyms

EC:3.1.3.16, BOR, BOP

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated EYA1 Polyclonal anti (CL488-22658)

    FC experiment of HeLa using CL488-22658

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Eya1 Polyclonal Antibody (CL488-22658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22658 targets EYA1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA1 fusion protein Ag18489 相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 1 (Drosophila)
Calculated molecular weight 592 aa, 65 kDa
Observed molecular weight 60 kDa, 50 kDa
GenBank accession numberBC121799
Gene symbol EYA1
Gene ID (NCBI) 2138
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 EYA1 antibody CL488-22658Download protocol
Standard Protocols
Click here to view our Standard Protocols