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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EYA4 Polyclonal antibody (24691-1-AP)

    WB analysis using 24691-1-AP (same clone as 24691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24691-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EYA4 Polyclonal antibody (24691-1-AP)

    WB analysis using 24691-1-AP (same clone as 24691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EYA4 Polyclonal antibody (24691-1-AP)

    IHC staining of human skeletal muscle using 24691-1-AP (same clone as 24691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EYA4 Polyclonal antibody (24691-1-AP)

    IHC staining of human skeletal muscle using 24691-1-AP (same clone as 24691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24691-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EYA4 Polyclonal antibody (24691-1-AP)

    IF Staining of HeLa using 24691-1-AP (same clone as 24691-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24691-1-AP (EYA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24691-1-PBS in a different storage buffer formulation.

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EYA4 Polyclonal antibody, PBS Only

EYA4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24691-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24691-1-PBS targets EYA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA4 fusion protein Ag20466 相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 4 (Drosophila)
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC041063
Gene Symbol EYA4
Gene ID (NCBI) 2070
RRIDAB_2879675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95677
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EYA4 (Eyes absent homolog 4) participates directly in the modulation of cellular signaling through its phosphatase activity and plays a role in the embryonic and post-natal development of the mature organs of CORTI. This protein may be involved in the development of the eye. It has 5 isoforms produced by alternative splicing.