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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Eg5 Recombinant antibody (84966-2-RR)

    WB analysis using 84966-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84966-2-RR (Eg5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Eg5 Recombinant antibody (84966-2-RR)

    IHC staining of human lung cancer using 84966-2-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84966-2-RR (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Recombinant antibody (84966-2-RR)

    IF Staining of HepG2 using 84966-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Eg5 Recombinant antibody (84966-2-RR)

    FC experiment of HeLa using 84966-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Eg5 Recombinant antibody (84966-2-RR, Clone:242611D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84966-2-RR

    Affinity and Kinetic Characterization of 84966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84966-2-RR against Human Eg5 were performed. The affinity constant is 18.3 nM.

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Eg5 Recombinant antibody

Eg5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84966-2-RR
Clone No.242611D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KIF11, 242611D10, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84966-2-RR targets Eg5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Eg5 fusion protein Ag20001 相同性解析による交差性が予測される生物種
Full Name kinesin family member 11
Calculated molecular weight 1056 aa, 119 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC136474
Gene Symbol Eg5
Gene ID (NCBI) 3832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52732
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Protocols

Product Specific Protocols
WB protocol for Eg5 antibody 84966-2-RRDownload protocol
IHC protocol for Eg5 antibody 84966-2-RRDownload protocol
IF protocol for Eg5 antibody 84966-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols