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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Eg5 Recombinant antibody, PBS Only (Capture)

Eg5 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242611D10

Cat no : 84966-2-PBS

Synonyms

KIF11, 242611D10, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Eg5 Recombinant antibody (84966-2-RR)

    WB analysis using 84966-2-RR (same clone as 84966-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84966-2-RR (Eg5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Recombinant antibody (84966-2-RR)

    IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Eg5 Recombinant antibody (84966-2-RR)

    FC experiment of HeLa using 84966-2-RR (same clone as 84966-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Eg5 Recombinant antibody (84966-2-RR, Clone:242611D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01688-2

    Cytometric bead array standard curve of MP01688-2

    Cytometric bead array standard curve of MP01688-2, Eg5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84966-2-PBS. Detection antibody: 84966-1-PBS. Standard: Ag20001. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84966-2-PBS targets Eg5 as part of a matched antibody pair:

MP01688-2: 84966-2-PBS capture and 84966-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Eg5 fusion protein Ag20001 相同性解析による交差性が予測される生物種
Full Name kinesin family member 11
Calculated molecular weight 1056 aa, 119 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC136474
Gene symbol Eg5
Gene ID (NCBI) 3832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.