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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

230047E8

Cat no : 82993-5-RR

Synonyms

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Endoglin/CD105 Recombinant antibody (82993-5-RR)

    IF Staining of HUVEC using 82993-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ENG antibody (82993-5-RR, Clone: 230047E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 82993-5-RR

    Affinity experiments of 82993-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82993-5-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.296 nM.

Tested Applications

Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82993-5-RR is Endoglin/CD105 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols