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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230047E8

Cat no : 82993-5-RR

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Endoglin/CD105 Recombinant antibody (82993-5-RR)

    IF Staining of HUVEC using 82993-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ENG antibody (82993-5-RR, Clone: 230047E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 82993-5-RR

    Affinity and Kinetic Characterization of 82993-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82993-5-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.296 nM.

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Tested Applications

Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82993-5-RR is Endoglin/CD105 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
RRIDAB_3670741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols