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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230047E8

Cat no : CL488-82993-5

Synonyms

CD105, 230047E8, HHT1, ENG, Endoglin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated Endoglin/CD105 Recom (CL488-82993-5)

    IF Staining of HUVEC using CL488-82993-5

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 Endoglin/CD105 antibody (CL488-82993-5, Clone: 230047E8 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82993-5 is Endoglin/CD105 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Endoglin/CD105 antibody CL488-82993-5Download protocol
Standard Protocols
Click here to view our Standard Protocols