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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26056

Synonyms

CVIL, CVL, Cytovillin, DKFZp762H157, EZR, ezrin, FLJ26216, p81, VIL2, Villin 2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-26056

    FC experiment of HeLa using CL488-26056

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Ezrin (CL488-26056) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Ezrin Ready-To-Use IHC Kit. Ezrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26056-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-26056 targets Ezrin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ezrin fusion protein Ag23530 相同性解析による交差性が予測される生物種
Full Name ezrin
Calculated molecular weight 69 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC013903
Gene symbol EZR
Gene ID (NCBI) 7430
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Ezrin antibody CL488-26056Download protocol
Standard Protocols
Click here to view our Standard Protocols