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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Coagulation Factor II Polyclonal antibody (24295-1-AP)

    WB analysis of human plasma using 24295-1-AP (same clone as 24295-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 24295-1-AP (Coagulation Factor II Antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24295-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Coagulation Factor II Polyclonal antibody (24295-1-AP)

    IP experiment of human plasma using 24295-1-AP (same clone as 24295-1-PBS)

    IP result of anti-Coagulation Factor II (IP:24295-1-AP, 4ug; Detection:24295-1-AP 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 24295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Coagulation Factor II Polyclonal antibody (24295-1-AP)

    IF Staining of HUVEC using 24295-1-AP (same clone as 24295-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 24295-1-AP (Coagulation Factor II antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24295-1-PBS in a different storage buffer formulation.

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Coagulation Factor II Polyclonal antibody, PBS Only

Coagulation Factor II Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24295-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Prothrombin, F2, PT, EC:3.4.21.5, Activation peptide fragment 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24295-1-PBS targets Coagulation Factor II in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Coagulation Factor II fusion protein Ag18385 相同性解析による交差性が予測される生物種
Full Name coagulation factor II (prothrombin)
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC051332
Gene Symbol F2
Gene ID (NCBI) 2147
RRIDAB_2879478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00734
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.