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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Factor VIII Monoclonal antibody (66722-1-Ig)

    WB analysis of human plasma using 66722-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66722-1-Ig (F8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human blood using Factor VIII Monoclonal antibody (66722-1-Ig)

    WB analysis of human blood using 66722-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66722-1-Ig (F8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Factor VIII Monoclonal antibody

Factor VIII Monoclonal Antibody for WB, ELISA
Cat No. 66722-1-Ig
Clone No.1C11E11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

F8, DXS1253E, Coagulation factor VIII, Antihemophilic factor, AHF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma tissue, human blood tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66722-1-Ig targets Factor VIII in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Factor VIII fusion protein Ag15772 相同性解析による交差性が予測される生物種
Full Name coagulation factor VIII, procoagulant component
Calculated molecular weight 2351 aa, 267 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022513
Gene Symbol F8
Gene ID (NCBI) 2157
RRIDAB_2882073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.

Protocols

Product Specific Protocols
WB protocol for Factor VIII antibody 66722-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols