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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Factor VIII Monoclonal antibody (66722-1-Ig)

    WB analysis of human plasma using 66722-1-Ig (same clone as 66722-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66722-1-Ig (F8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Factor VIII Monoclonal antibody (66722-1-Ig)

    WB analysis of human blood using 66722-1-Ig (same clone as 66722-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66722-1-Ig (F8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66722-1-PBS in a different storage buffer formulation.

Factor VIII Monoclonal antibody, PBS Only

Factor VIII Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66722-1-PBS
Clone No.1C11E11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

F8, DXS1253E, Coagulation factor VIII, Antihemophilic factor, AHF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66722-1-PBS targets Factor VIII in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15772

Product name: Recombinant human F8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2144-2351 aa of BC022513

Sequence: VFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

相同性解析による交差性が予測される生物種
Full Name coagulation factor VIII, procoagulant component
Calculated molecular weight 2351 aa, 267 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022513
Gene Symbol F8
Gene ID (NCBI) 2157
RRIDAB_2882073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP00451
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.

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