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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAAH Polyclonal antibody (17909-1-AP)

    WB analysis using 17909-1-AP (same clone as 17909-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using FAAH Polyclonal antibody (17909-1-AP)

    WB analysis of A431 using 17909-1-AP (same clone as 17909-1-PBS)

    WB result of FAAH antibody (17909-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAAH transfected A431 cells. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using FAAH Polyclonal antibody (17909-1-AP)

    WB analysis of mouse liver using 17909-1-AP (same clone as 17909-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using FAAH Polyclonal antibody (17909-1-AP)

    WB analysis of rat testis using 17909-1-AP (same clone as 17909-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using FAAH Polyclonal antibody (17909-1-AP)

    WB analysis of A431 using 17909-1-AP (same clone as 17909-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FAAH Polyclonal antibody (17909-1-AP)

    IP experiment of mouse liver using 17909-1-AP (same clone as 17909-1-PBS)

    IP result of anti-FAAH (IP:17909-1-AP, 4ug; Detection:17909-1-AP 1:500) with mouse liver tissue lysate 6400 ug. This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of mouse testis using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of mouse testis using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of human placenta using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of human placenta using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of human testis using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FAAH Polyclonal antibody (17909-1-AP)

    IHC staining of human testis using 17909-1-AP (same clone as 17909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17909-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FAAH Polyclonal antibody, PBS Only

FAAH Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17909-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17909-1-PBS targets FAAH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAAH fusion protein Ag12304 相同性解析による交差性が予測される生物種
Full Name fatty acid amide hydrolase
Calculated molecular weight 579 aa, 63 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC093632
Gene Symbol FAAH
Gene ID (NCBI) 2166
RRIDAB_2878465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00519
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.