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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6C8

Cat no : CL488-68227

Synonyms

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite® Plus 488-conjugated FABP1 Monoclonal ant (CL488-68227)

    IF Staining of mouse small intestine using CL488-68227

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 FABP1 antibody (CL488-68227, Clone: 1B6C8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated FABP1 Monoclonal ant (CL488-68227)

    FC experiment of HepG2 using CL488-68227

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated FABP1 Monoclonal antibody (CL488-68227, Clone:1B6C8)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse small intestine tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68227 targets FABP1 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP1 fusion protein Ag32836 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 1, liver
Calculated molecular weight 127 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC032801
Gene symbol FABP1
Gene ID (NCBI) 2168
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FABP1 antibody CL488-68227Download protocol
FC protocol for CL Plus 488 FABP1 antibody CL488-68227Download protocol
Standard Protocols
Click here to view our Standard Protocols