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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated FABP5 Polyclonal antibody

FABP5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-12348

Synonyms

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated FABP5 Polyclonal antibody (CL555-12348)

    IF Staining of HeLa using CL555-12348

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 FABP5 antibody (CL555-12348) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated FABP5 Polyclonal antibody (CL555-12348)

    IF Staining of HeLa using CL555-12348

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 FABP5 antibody (CL555-12348) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated FABP5 Polyclonal antibody (CL555-12348)

    FC experiment of HeLa using CL555-12348

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human FABP5 (CL555-12348) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-12348 targets FABP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FABP5 fusion protein Ag3005 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 5 (psoriasis-associated)
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC019385
Gene symbol FABP5
Gene ID (NCBI) 2171
RRIDAB_2919608
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Protocols

Product Specific Protocols
IF protocol for CL555 FABP5 antibody CL555-12348Download protocol
Standard Protocols
Click here to view our Standard Protocols