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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig (same clone as 66629-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66629-1-Ig (FAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig (same clone as 66629-1-PBS)

    WB result of FAF2 antibody (66629-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells. This data was developed using the same antibody clone with 66629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig (same clone as 66629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig (same clone as 66629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of mouse brain using 66629-1-Ig (same clone as 66629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66629-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FAF2 Monoclonal antibody, PBS Only

FAF2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66629-1-PBS
Clone No.3H6D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66629-1-PBS targets FAF2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAF2 fusion protein Ag21536 相同性解析による交差性が予測される生物種
Full Name Fas associated factor family member 2
Calculated molecular weight 445 aa, 53 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
RRIDAB_2881989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CS3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.