• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66629-1-Ig (FAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig

    WB result of FAF2 antibody (66629-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of mouse brain using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66629-1-Ig
Clone No.3H6D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66629-1-Ig targets FAF2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAF2 fusion protein Ag21536 相同性解析による交差性が予測される生物種
Full Name Fas associated factor family member 2
Calculated molecular weight 445 aa, 53 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
RRIDAB_2881989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

Protocols

Product Specific Protocols
WB protocol for FAF2 antibody 66629-1-IgDownload protocol
IHC protocol for FAF2 antibody 66629-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols