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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H6D2

Cat no : CL488-66629

Synonyms

ETEA, 3H6D2, Protein ETEA, KIAA0887, FAS-associated factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated FAF2 Monoclonal anti (CL488-66629)

    WB analysis using CL488-66629

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66629 (FAF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66629 targets FAF2 in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAF2 fusion protein Ag21536 相同性解析による交差性が予測される生物種
Full Name Fas associated factor family member 2
Calculated molecular weight 445 aa, 53 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC014001
Gene symbol FAF2
Gene ID (NCBI) 23197
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 FAF2 antibody CL488-66629Download protocol
Standard Protocols
Click here to view our Standard Protocols