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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAH Monoclonal antibody (67986-1-Ig)

    WB analysis using 67986-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of pig liver tissue using FAH Monoclonal antibody (67986-1-Ig)

    WB analysis of pig liver using 67986-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using FAH Monoclonal antibody (67986-1-Ig)

    WB analysis of rat liver using 67986-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FAH Monoclonal antibody (67986-1-Ig)

    WB analysis of mouse liver using 67986-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAH Monoclonal antibody (67986-1-Ig)

    IF Staining of HepG2 using 67986-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAH Monoclonal antibody (67986-1-Ig)

    IF Staining of HepG2 using 67986-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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FAH Monoclonal antibody

FAH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67986-1-Ig
Clone No.2F8E11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Fumarylacetoacetate hydrolase, FAA, EC:3.7.1.2, Beta-diketonase, 2F8E11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, pig liver tissue, rat liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67986-1-Ig targets FAH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAH fusion protein Ag6704 相同性解析による交差性が予測される生物種
Full Name fumarylacetoacetate hydrolase (fumarylacetoacetase)
Calculated molecular weight 46 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC002527
Gene Symbol FAH
Gene ID (NCBI) 2184
RRIDAB_2918735
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).

Protocols

Product Specific Protocols
WB protocol for FAH antibody 67986-1-IgDownload protocol
IF protocol for FAH antibody 67986-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols