Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67986 targets FAH in IF/ICC applications and shows reactivity with Human, pig, rat, mouse samples.
| Tested Reactivity | Human, pig, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FAH fusion protein Ag6704 相同性解析による交差性が予測される生物種 |
| Full Name | fumarylacetoacetate hydrolase (fumarylacetoacetase) |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC002527 |
| Gene Symbol | FAH |
| Gene ID (NCBI) | 2184 |
| RRID | AB_2934605 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16930 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FAH antibody CL488-67986 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |