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FAK Monoclonal antibody

FAK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66258-1-Ig
Clone No.2C5B9

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhTERT-RPE1 cells, human testis tissue, MCF-7 cells, HCT 116 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66258-1-Ig targets FAK in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen FAK fusion protein Ag17966 相同性解析による交差性が予測される生物種
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene Symbol FAK
Gene ID (NCBI) 5747
RRIDAB_2881646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protocols

Product Specific Protocols
WB protocol for FAK antibody 66258-1-IgDownload protocol
IHC protocol for FAK antibody 66258-1-IgDownload protocol
IF protocol for FAK antibody 66258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

Lipid-anchored proteasomes control membrane protein homeostasis

Authors - Ruizhu Zhang
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma
humanWB

Cancer Lett

Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma

Authors - Qianyu Shi
humanWB

Oncogene

Rab34 regulates adhesion, migration, and invasion of breast cancer cells.

Authors - Lixiang Sun
humanWB

Oncogene

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.

Authors - Ran-Ran Ma
humanWB

Aging (Albany NY)

TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.

Authors - Jiao Wu
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