FAK Monoclonal antibody, PBS Only

WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Western blot analysis of FAK-specific in various cell lines using Proteintech antibody 66258-1-Ig at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

human testis tissue were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.