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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-FAK (Tyr397) Recombinant antibody

Phospho-FAK (Tyr397) Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240630F8

Cat no : 83933-1-RR

Synonyms

p FAK, p-FAK, Pho FAK, Pho-FAK, Phospho FAK Tyr397

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-FAK (Tyr397) Recombinant antibody (83933-1-RR)

    WB analysis using 83933-1-RR

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Phospho-FAK (Tyr397) Recombinant antibody (83933-1-RR)

    IF Staining of A549 using 83933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Phospho-FAK (Tyr397) Recombinant antibody (83933-1-RR)

    IF Staining of A549 using 83933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inpervanadate treated HeLa cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83933-1-RR targets Phospho-FAK (Tyr397) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene symbol FAK
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.

Protocols

Product Specific Protocols
WB protocol for Phospho-FAK (Tyr397) antibody 83933-1-RRDownload protocol
IF protocol for Phospho-FAK (Tyr397) antibody 83933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols