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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Phospho-FAK (Tyr397) Recombinant antibody

Phospho-FAK (Tyr397) Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240630F8

Cat no : CL488-83933

Synonyms

FAK, PTK2, Focal adhesion kinase 1, FAK1, FADK 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated Phospho-FAK (Tyr397) (CL488-83933)

    IF Staining of A549 using CL488-83933

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Phospho-FAK (Tyr397) antibody (CL488-83933, Clone: 240630F8 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83933 targets Phospho-FAK (Tyr397) in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene symbol FAK
Gene ID (NCBI) 5747
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-FAK (Tyr397) antibody CL488-83933Download protocol
Standard Protocols
Click here to view our Standard Protocols