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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67926 targets FAM114A1 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FAM114A1 fusion protein Ag17289 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 114, member A1 |
| Calculated molecular weight | 563 aa, 61 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC040452 |
| Gene Symbol | FAM114A1 |
| Gene ID (NCBI) | 92689 |
| RRID | AB_3084384 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWE2 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FAM114A1 antibody CL488-67926 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |