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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM3C Monoclonal antibody (60282-1-Ig)

    IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM3C antibody (60282-1-Ig, Clone: 2E10D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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FAM3C Monoclonal antibody, PBS Only

FAM3C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60282-1-PBS
Clone No.2E10D5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein FAM3C, Interleukin-like EMT inducer, Interleukin like EMT inducer, ILEI, GS3786

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60282-1-PBS targets FAM3C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAM3C fusion protein Ag5516 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 3, member C
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC046932
Gene Symbol FAM3C
Gene ID (NCBI) 10447
RRIDAB_2881400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92520
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.