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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM3C Monoclonal antibody

FAM3C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E10D5

Cat no : 60282-1-Ig

Synonyms

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    WB analysis of fetal human brain using 60282-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    IHC staining of human oesophagus cancer using 60282-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM3C Monoclonal antibody (60282-1-Ig)

    IHC staining of human oesophagus cancer using 60282-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM3C Monoclonal antibody (60282-1-Ig)

    IF Staining of HepG2 using 60282-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60282-1-Ig(FAM3C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60282-1-Ig targets FAM3C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAM3C fusion protein Ag5516 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 3, member C
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC046932
Gene symbol FAM3C
Gene ID (NCBI) 10447
RRIDAB_2881400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Protocols

Product Specific Protocols
WB protocol for FAM3C antibody 60282-1-IgDownload protocol
IHC protocol for FAM3C antibody 60282-1-IgDownload protocol
IF protocol for FAM3C antibody 60282-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols