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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated FAM3C Monoclonal antibody (CL594-60282)

    IF Staining of HepG2 using CL594-60282

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60282 (FAM3C antibody) at dilution of 1:100.

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CoraLite®594-conjugated FAM3C Monoclonal antibody

FAM3C Monoclonal Antibody for IF/ICC
Cat No. CL594-60282
Clone No.2E10D5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60282 targets FAM3C in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAM3C fusion protein Ag5516 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 3, member C
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC046932
Gene Symbol FAM3C
Gene ID (NCBI) 10447
RRIDAB_2883453
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92520
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Protocols

Product Specific Protocols
IF protocol for CL594 FAM3C antibody CL594-60282Download protocol
Standard Protocols
Click here to view our Standard Protocols