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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FANCD2 Recombinant antibody (83946-4-RR)

    WB analysis using 83946-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCD2 Recombinant antibody (83946-4-RR)

    IF Staining of HeLa using 83946-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCD2 antibody (83946-4-RR, Clone: 241048G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin.

  • Flow cytometry (FC) experiment of HeLa cells using FANCD2 Recombinant antibody (83946-4-RR)

    FC experiment of HeLa using 83946-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83946-4-RR

    Affinity and Kinetic Characterization of 83946-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

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FANCD2 Recombinant antibody

FANCD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83946-4-RR
Clone No.241048G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241048G8, FA4, FACD, FAD, FAD2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, Jurkat cells, U2OS cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83946-4-RR targets FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FANCD2 fusion protein Ag29460 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group D2
Calculated molecular weight 164 kDa
Observed molecular weight150-160 kDa
GenBank accession numberNM_001018115
Gene Symbol FANCD2
Gene ID (NCBI) 2177
RRIDAB_3671526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

Protocols

Product Specific Protocols
WB protocol for FANCD2 antibody 83946-4-RRDownload protocol
IF protocol for FANCD2 antibody 83946-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols