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  • Featured Product
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FARSB Monoclonal antibody

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10C2

Cat no : 67924-1-Ig

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HepG2 using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells.

  • Western Blot (WB) analysis of HeLa cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HeLa using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HEK-293 using 67924-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse brain using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse brain using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FARSB Monoclonal antibody (67924-1-Ig)

    IF Staining of HeLa using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using FARSB Monoclonal antibody (67924-1-Ig)

    IF Staining of U-251 using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67924-1-Ig targets FARSB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FARSB fusion protein Ag9695 相同性解析による交差性が予測される生物種
Full Name phenylalanyl-tRNA synthetase, beta subunit
Calculated molecular weight 589 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC017783
Gene symbol FARSB
Gene ID (NCBI) 10056
RRIDAB_2918676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FARSB antibody 67924-1-IgDownload protocol
IHC protocol for FARSB antibody 67924-1-IgDownload protocol
IF protocol for FARSB antibody 67924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

Nan Fang Yi Ke Da Xue Xue Bao

[FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]

Authors - Ziran Zhang
View Article