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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FARSB Monoclonal antibody

FARSB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H10C2

Cat no : CL594-67924

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite®594-conjugated FARSB Monoclonal antibody (CL594-67924)

    IF Staining of U-251 using CL594-67924

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite®594 FARSB antibody (CL594-67924, Clone: 1H10C2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite®594-conjugated FARSB Monoclonal antibody (CL594-67924)

    IF Staining of U-251 using CL594-67924

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite®594 FARSB antibody (CL594-67924, Clone: 1H10C2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-251 cells using CoraLite®594-conjugated FARSB Monoclonal antibody (CL594-67924)

    FC experiment of U-251 using CL594-67924

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated FARSB Monoclonal antibody (CL594-67924, Clone:1H10C2)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67924 targets FARSB in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FARSB fusion protein Ag9695 相同性解析による交差性が予測される生物種
Full Name phenylalanyl-tRNA synthetase, beta subunit
Calculated molecular weight 589 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC017783
Gene symbol FARSB
Gene ID (NCBI) 10056
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 FARSB antibody CL594-67924Download protocol
FC protocol for CL594 FARSB antibody CL594-67924Download protocol
Standard Protocols
Click here to view our Standard Protocols