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CoraLite® Plus 488-conjugated FBF1 Polyclonal antibody
FBF1 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11531
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A549 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11531 targets FBF1 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBF1 fusion protein Ag2106 相同性解析による交差性が予測される生物種 |
| Full Name | Fas (TNFRSF6) binding factor 1 |
| Calculated molecular weight | 1133 aa, 125 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC023549 |
| Gene symbol | FBF1 |
| Gene ID (NCBI) | 85302 |
| RRID | AB_2919031 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FBF1 antibody CL488-11531 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Karine (Verified Customer) (02-26-2024) | FBF1-Coralite488 (in green) does label one extremity of the primary cilium labeled with ARL13B (in red) in RPE1 cells whose nuclei appear in purple and cell contour in grey with Brightfield. It is quite specific when used at 1/1000 overnight at 4°C. There is a bit more aspecific signal than with the uncoupled antibdy but it remains quite reasonable.
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