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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBL Monoclonal antibody (66985-1-Ig)

    WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FBL Monoclonal antibody (66985-1-Ig)

    WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using FBL Monoclonal antibody (66985-1-Ig)

    IP experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    IP result of anti-FBL (IP:66985-1-Ig, 5ug; Detection:66985-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FBL Monoclonal antibody (66985-1-Ig)

    IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FBL Monoclonal antibody (66985-1-Ig)

    IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBL Monoclonal antibody (66985-1-Ig)

    IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FBL Monoclonal antibody (66985-1-Ig)

    IF Staining of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBL Monoclonal antibody (66985-1-Ig)

    IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using FBL Monoclonal antibody (66985-1-Ig)

    FC experiment of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FBL Monoclonal antibody (66985-1-Ig)

    FC experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FBL Monoclonal antibody (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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FBL Monoclonal antibody, PBS Only

FBL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 66985-1-PBS
Clone No.3A9E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66985-1-PBS targets FBL in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
RRIDAB_2882303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22087
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).