FBP1 Monoclonal antibody
FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2G4E4
Cat no : 68446-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, MCF-7 cells, T-47D cells, pig liver tissue, rat liver tissue |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:40000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68446-1-Ig targets FBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | FBP1 fusion protein Ag3837 相同性解析による交差性が予測される生物種 |
Full Name | fructose-1,6-bisphosphatase 1 |
Calculated molecular weight | 338 aa, 37 kDa |
Observed molecular weight | 37-40 kDa |
GenBank accession number | BC012927 |
Gene symbol | FBP1 |
Gene ID (NCBI) | 2203 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).
Protocols
Product Specific Protocols | |
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WB protocol for FBP1 antibody 68446-1-Ig | Download protocol |
IHC protocol for FBP1 antibody 68446-1-Ig | Download protocol |
IF protocol for FBP1 antibody 68446-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |