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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of T-47D using 68446-1-Ig (same clone as 68446-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of pig liver using 68446-1-Ig (same clone as 68446-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FBP1 Monoclonal antibody (68446-1-Ig)

    IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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FBP1 Monoclonal antibody, PBS Only

FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68446-1-PBS
Clone No.2G4E4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68446-1-PBS targets FBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FBP1 fusion protein Ag3837 相同性解析による交差性が予測される生物種
Full Name fructose-1,6-bisphosphatase 1
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC012927
Gene Symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09467
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).