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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXL4 Polyclonal antibody (24754-1-AP)

    WB analysis using 24754-1-AP (same clone as 24754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24754-1-AP (FBXL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24754-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FBXL4 Polyclonal antibody (24754-1-AP)

    IF Staining of U2OS using 24754-1-AP (same clone as 24754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXL4 antibody (24754-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24754-1-PBS in a different storage buffer formulation.

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FBXL4 Polyclonal antibody, PBS Only

FBXL4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24754-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FBL5, FBL4, F box/LRR repeat protein 4, F box protein FBL4/FBL5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24754-1-PBS targets FBXL4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL4 fusion protein Ag21579 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 4
Calculated molecular weight 621 aa, 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC055010
Gene Symbol FBXL4
Gene ID (NCBI) 26235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKA2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FBXL4 (F-box and leucine-rich repeat protein 4) is a mitochondrial protein that has been recently found to play significant roles in mtDNA maintenance (PMID: 28940506). Moreover, FBXL4 is a substrate receptor of a Cullin-1-RING E3 (CRL1) ligase complex that has previously been shown to bind to SKP1, the common CRL1 adapter subunit (PMID: 37102372).