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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FBXO22 Monoclonal antibody (68650-1-Ig)

    WB analysis of Jurkat using 68650-1-Ig

    WB result of FBXO22 antibody (68650-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and FBXO22 knockout Jurkat cells.

  • Western Blot (WB) analysis of various lysates using FBXO22 Monoclonal antibody (68650-1-Ig)

    WB analysis using 68650-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68650-1-Ig (FBXO22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

FBXO22 Monoclonal antibody

FBXO22 Monoclonal Antibody for WB, ELISA
Cat No. 68650-1-Ig
Clone No.4B8A12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

FBX22, F-box protein FBX22p44, F-box only protein 22, F box protein 22, F box only protein 22

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68650-1-Ig targets FBXO22 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBXO22 fusion protein Ag5294 相同性解析による交差性が予測される生物種
Full Name F-box protein 22
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC039024
Gene Symbol FBXO22
Gene ID (NCBI) 26263
RRIDAB_3670392
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NEZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Protocols

Product Specific Protocols
WB protocol for FBXO22 antibody 68650-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols