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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FBXO32 Monoclonal antibody

FBXO32 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C2G3

Cat no : CL488-67172

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated FBXO32 Monoclonal an (CL488-67172)

    IF Staining of mouse skeletal muscle using CL488-67172

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 FBXO32 antibody (CL488-67172, Clone: 3C2G3 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67172 targets FBXO32 in IF-P applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FBXO32 fusion protein Ag25247 相同性解析による交差性が予測される生物種
Full Name F-box protein 32
Calculated molecular weight 355 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC024030
Gene symbol FBXO32
Gene ID (NCBI) 114907
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FBXO32 antibody CL488-67172Download protocol
Standard Protocols
Click here to view our Standard Protocols