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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FBXO44 Recombinant antibody

FBXO44 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230229G7

Cat no : CL488-82857-2

Synonyms

FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated FBXO44 Recombinant a (CL488-82857-2)

    IF Staining of A431 using CL488-82857-2

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 FBXO44 antibody (CL488-82857-2, Clone: 230229G7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated FBXO44 Recombinant a (CL488-82857-2)

    FC experiment of A431 using CL488-82857-2

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fbxo44 Recombinant Antibody (CL488-82857-2, Clone:230229G7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82857-2 targets FBXO44 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FBXO44 fusion protein Ag0977 相同性解析による交差性が予測される生物種
Full Name F-box protein 44
Calculated molecular weight 26 kDa
Observed molecular weight30 kDa
GenBank accession numberBC007832
Gene symbol FBXO44
Gene ID (NCBI) 93611
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FBXO44 antibody CL488-82857-2Download protocol
FC protocol for CL Plus 488 FBXO44 antibody CL488-82857-2Download protocol
Standard Protocols
Click here to view our Standard Protocols