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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO5 Polyclonal antibody, PBS Only

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10872-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HEK-293 using 10872-1-AP (same clone as 10872-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of K-562 using 10872-1-AP (same clone as 10872-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HeLa using 10872-1-AP (same clone as 10872-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HepG2 using 10872-1-AP (same clone as 10872-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of mouse kidney using 10872-1-AP (same clone as 10872-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of rat liver using 10872-1-AP (same clone as 10872-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ovary tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of human ovary using 10872-1-AP (same clone as 10872-1-PBS)

    human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10872-1-PBS targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO5 fusion protein Ag1296 相同性解析による交差性が予測される生物種
Full Name F-box protein 5
Calculated molecular weight 54 kDa
Observed molecular weight 56 kDa, 45 kDa
GenBank accession numberBC018905
Gene symbol FBXO5
Gene ID (NCBI) 26271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.