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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    IF Staining of mouse spleen using 30356-1-AP (same clone as 30356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FCGR3A/CD16a antibody (30356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

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FCGR3A/CD16a Polyclonal antibody, PBS Only

FCGR3A/CD16a Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 30356-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Synonyms

CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30356-1-PBS targets FCGR3A/CD16a in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR3A/CD16a fusion protein Ag1281 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 29 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC036723
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.