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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FDX1 Recombinant antibody

FDX1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230196B12

Cat no : CL488-82957

Synonyms

Ferredoxin-1, FDX, ADX, 230196B12

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated FDX1 Recombinant ant (CL488-82957)

    IF Staining of HepG2 using CL488-82957

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FDX1 antibody (CL488-82957, Clone: 230196B12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated FDX1 Recombinant ant (CL488-82957)

    FC experiment of A431 using CL488-82957

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fdx1 Recombinant Antibody (CL488-82957, Clone:230196B12)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated FDX1 Recombinant ant (CL488-82957)

    FC experiment of HepG2 using CL488-82957

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fdx1 Recombinant Antibody (CL488-82957, Clone:230196B12)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82957 targets FDX1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDX1 fusion protein Ag3301 相同性解析による交差性が予測される生物種
Full Name ferredoxin 1
Calculated molecular weight 184 aa, 19 kDa
Observed molecular weight14 kDa
GenBank accession numberBC017063
Gene symbol FDX1
Gene ID (NCBI) 2230
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FDX1 antibody CL488-82957Download protocol
FC protocol for CL Plus 488 FDX1 antibody CL488-82957Download protocol
Standard Protocols
Click here to view our Standard Protocols