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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FER Monoclonal antibody

FER Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3H2

Cat no : 67671-1-Ig

Synonyms

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FER Monoclonal antibody (67671-1-Ig)

    WB analysis using 67671-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of ROS1728 cells using FER Monoclonal antibody (67671-1-Ig)

    WB analysis of ROS1728 using 67671-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using FER Monoclonal antibody (67671-1-Ig)

    WB analysis of 4T1 using 67671-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using FER Monoclonal antibody (67671-1-Ig)

    IF Staining of NIH/3T3 using 67671-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using FER antibody (67671-1-Ig, Clone: 1E3H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, ROS1728 cells, 4T1 cells, HEK-293 cells, Hela cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67671-1-Ig targets FER in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FER fusion protein Ag19176 相同性解析による交差性が予測される生物種
Full Name fer (fps/fes related) tyrosine kinase
Calculated molecular weight 822 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC017060
Gene symbol FER
Gene ID (NCBI) 2241
RRIDAB_2882867
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FER antibody 67671-1-IgDownload protocol
IF protocol for FER antibody 67671-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols